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6月に米国サンディエゴにて開催されたCell Line Development & Engineering 2017学会において、CloneSelect Imager FLを使用したモノクローナリティ（単クローン性）の高精度な評価についてポスター展示を行いました。CloneSelect Imagerは従来の高速な透過光画像の取得に加え、蛍光画像取得機能を有し、より正確なモノクローナリティの評価を可能とすることで細胞株樹立のプロセスを促進します。

A High Fidelity Monoclonality Verification Method to Boost Cell Line Developmen

Summary: Bio-therapeutic production is a highly regulated process that relies on the generation of a cell line expressing the gene of interest from a single clonal population of cells. Providing evidence that a cell line is derived from a single cell is a crucial factor in cell line generation; however, a major challenge in this process is at the level of selecting single cells with the desired recombinant qualities. Here, we demonstrate a method to isolate single-cell derived clonal populations with high confidence based on fluorescence labeling of single cell-containing wells at day 0. The CloneSelect Imager FL from Molecular Devices allows for automated analysis of the fluorescence and white light images to provide cell counts for each well. Users can view both the white light and fluorescence images to confirm the results and follow the timeline of growth from a single cell to a colony. Single cell identification with the aid of fluorescence offers a more conclusive method to meet stringent regulatory demands in cell line development.